Leukocyte and platelet rich fibrin (L-PRF) is a second-generation autologous chairside preparation of platelet concentrate derived from whole venous blood. It is obtained through a specific process of gradient density centrifugation that limits the degree of blood manipulation without requiring the use of a platelet activator. L-PRF produces a dense fibrin matrix that incorporates leukocytes, platelets, and high concentrations of growth factors. When compressed, the resulting fibrin matrix is strong, pliable, and can be sutured for use as a stand-alone product to improve wound healing and promote tissue regeneration. The matrix can also be incorporated into particulate bone graft materials or other biomaterials to improve both the handling characteristics and the healing properties of the particulate material. The use of leukocyte and platelet rich fibrin immediately following a procedure can reduce the swelling and discomfort experienced by the patient while also speeding the regeneration, wound healing, and recovery processes.